COMPLEX BETWEEN NC10 ANTI-INFLUENZA VIRUS NEURAMINIDASE SINGLE CHAIN ANTIBODY WITH A 5 RESIDUE LINKER AND INFLUENZA VIRUS NEURAMINIDASE


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B-Cell Epitope
Epitope  
   
Complex PDB ID 1A14
Accession Number 3DIEP0005
IEDB ID 77486
Epitope Sequence P330; N331; D332; P333; T334; Y342; P343; G344; N345; I367; I369; A370; S371; N400; T401; W403; K434
Starting Position330-334,342-345,367,369
Ending Position371,400-401,403
Epitope Type Discontinuous Residues

Assay Information  
Assay Antigen Neuraminidase Neuraminidase Influenza A virus (A/tern/Australia/G70C/1975(H11N9))
PDB CategoryCOMPLEX (ANTIBODY/ANTIGEN)
Keyword COMPLEX (ANTIBODY/ANTIGEN); SINGLE-CHAIN ANTIBODY; GLYCOSYLATED PROTEIN
Antibody Residues Interacting with Antigen Open in new window      Download dimplot pdb file
Antibody Chain 1 PDB Chain H
Antibody Chain 2 PDB ChainL
Antigen PDB ChainN
Contact Area for Antigen 683.3
Contact Area for Antibody685.4
CommentsIn the assay recombinant single-chain Fv construct of antibody NC10 with a five-residue peptide linker between VH and VL (scFv(5)) and tetrameric neuraminidase were used.

Experimental Details
Method
X-RAY DIFFRACTION
Resolution
2.5
R-Value
0.2
Space Group
I 4 2 2
Unit Cell
Length(Å) Angle(°)
a = 165.3 α = 90
b = 165.3 β = 90
c = 182.4 γ = 90


Source Information  
Structure Determination Method X-RAY DIFFRACTION
Source Organism Influenza A virus
Taxonomic ID11320
Entity ID1

Ligand non-polymer
Ligand NameN-ACETYL-D-GLUCOSAMINE
Chem Name NAG
Ligand NameBETA-D-MANNOSE
Chem Name BMA
Ligand NameALPHA-D-MANNOSE
Chem Name MAN
Ligand Name2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE
Chem Name NDG
Ligand NameCALCIUM ION
Chem Name CA




Sequence
Protein Name
CALCIUM ION
Poly type
polypeptide(L)
Sequence status
Complete

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