TAQ POLYMERASE IN COMPLEX WITH TP7; AN INHIBITORY FAB


Tab-View Sample


B-Cell Epitope
Epitope  
   
Complex PDB ID 1BGX
Accession Number 3DIEP0137
IEDB ID 77382
Epitope Sequence R313; K314; S383; T385; T386; E388; E397; W398; E471; P481; L484; N485; K511; R512; S515; R536; K542; S543; T544; Y545; I546; D547; P548; A568; A570; N580; R728
Starting Position313-314,383,385-386,388,397-398,471,481,484-485,511-512,515,536,542-548,568,570,580,728
Ending Position728
Epitope Type Discontinuous Epitope

Assay Information  
Assay Antigen MRGMLPLFEPKGRVL LVDGHHLAYRTFHAL KGLTTSRGEPVQAVY GFAKS… DNA polymerase I; thermostable (1-832) Thermus aquaticus
PDB CategoryCOMPLEX (POLYMERASE/INHIBITOR)
Keyword DNA POLYMERASE; FAB; PCR; INHIBITION; HELIX-COIL DYNAMICS; INHIBITOR DESIGN; COMPLEX (POLYMERASE/INHIBITOR)
Antibody Residues Interacting with Antigen Open in new window      Download dimplot pdb file
Antibody Chain 1 PDB Chain H
Antibody Chain 2 PDB ChainL
Antigen PDB ChainT
Contact Area for Antigen 2952.1
Contact Area for Antibody2861.6
CommentsAn inhibitory activity of TP7 Fab is directly attributable to its interference with DNA binding by the polymerase domain of the enzyme. Fab binds the region of TaqP involved in DNA binding.

Experimental Details
Method
X-RAY DIFFRACTION
Resolution
2.3
R-Value
0.189
Space Group
P 1
Unit Cell
Length(Å) Angle(°)
a = 76.6 α = 100.7
b = 89.1 β = 115.3
c = 89.3 γ = 95.3


Source Information  
Structure Determination Method X-RAY DIFFRACTION

Ligand




Sequence
Protein Name
TAQ POLYMERASE IN COMPLEX WITH TP7; AN INHIBITORY FAB
Poly type
polypeptide(L)
Sequence status
Complete

Err:Conn