CRYSTAL STRUCTURE OF THE ANTI-LYSOZYME ANTIBODY HYHEL-63 COMPLEXED WITH HEN EGG WHITE LYSOZYME


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B-Cell Epitope
Epitope  
   
Complex PDB ID 1DQJ
Accession Number 3DIEP0025
IEDB ID 135872
Epitope Sequence R32; H33; G34; D36; N37; Y38; R39; W80; W81; R91; L93; N95; T107; N111; K114; K115; I116 S118; D119; G120; N121
Starting Position32-34,36-39,80-81,91,93,95,107,111,114-
Ending Position116,118-121
Epitope Type Discontinuous Epitope

Assay Information  
Assay Antigen Lysozyme C precursor Gallus gallus
PDB Categoryimmune system/hydrolase
Keyword antibody; protein-protein complex; hen egg white lysozyme; immune system/hydrolase COMPLEX
Antibody Residues Interacting with Antigen Open in new window      Download dimplot pdb file
Antibody Chain 1 PDB Chain B
Antibody Chain 2 PDB ChainA
Antigen PDB ChainC
Contact Area for Antigen 902.8
Contact Area for Antibody861.8
CommentsIn comparison with the structure of free Fab HyHEL-63 [PDB: 1DQQ; 1DQM]; significant conformational changes; that can be attributed to the antigen binding rather than arise from crystallographic contacts; were observed at the tip of CDR H3 (residues B: Tyr98; Gly99 ) and in the CDR H2 residues 53-56. A comparison of the structures of the bound to Fab HyHEL-63 (this structure) and free HEL in two crystal forms [PDB: 1HEL; 2LZT] revealed the major conformational changes at Asp101 and Gly102 residues.

Experimental Details
Method
X-RAY DIFFRACTION
Resolution
2
R-Value
0.217
Space Group
P 42 21 2
Unit Cell
Length(Å) Angle(°)
a = 90.58 α = 90
b = 90.58 β = 90
c = 150.06 γ = 90


Source Information  
Structure Determination Method X-RAY DIFFRACTION


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Ligand
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