CRYSTAL STRUCTURE OF PHAGE LIBRARY-DERIVED SINGLE-CHAIN FV FRAGMENT 1F9 IN COMPLEX WITH TURKEY EGG-WHITE LYSOZYME


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B-Cell Epitope
Epitope  
   
Complex PDB ID 1DZB
Accession Number 3DIEP0239
IEDB ID 135873
Epitope Sequence R39; Y41; W80; W81; K91; L93; K115; I116; S118; G119; G120; N121; N124; L134
Starting Position39,41,80-81,91,93,115,116,117-124,134
Ending Position134
Epitope Type Discontinuous Epitope

Assay Information  
Assay Antigen Lysozyme C Meleagris gallopavo
PDB CategoryIMMUNE SYSTEM
Keyword IMMUNE SYSTEM; COMPLEX (ANTIBODY ANTIGEN); SINGLE-DOMAIN ANTIBODY; TURKEY EGG-WHITE LYSOZYME; ANTIBODY- PROTEIN COMPLEX; SINGLE-CHAIN FV FRAGMENT
Antibody Residues Interacting with Antigen Open in new window      Download dimplot pdb file
Antibody Chain 1 PDB Chain B
Antibody Chain 2 PDB ChainB
Antigen PDB ChainY
Contact Area for Antigen 863.8
Contact Area for Antibody774.9
CommentsThe epitope of scFv 1F9 on turkey egg-white lysozyme was determined from the X-ray structure of the complex. The asymmetric unit contains two scFv/TEL complexes with crystallographic contacts between the two scFv molecules. The paratopes and contacts are slightly different in the complexes; therefore both were curated. This assay is for the complex 1 formed by PDB chains A and X.

Experimental Details
Method
X-RAY DIFFRACTION
Resolution
2
R-Value
Space Group
P 1 21 1
Unit Cell
Length(Å) Angle(°)
a = 38.71 α = 90
b = 112.44 β = 97.67
c = 80 γ = 90



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Source Information  
Structure Determination Method X-RAY DIFFRACTION
Source Organism MUS MUSCULUS
Common NameHOUSE MOUSE
Taxonomic ID10090
Source Organism MELEAGRIS GALLOPAVO
Common NameCOMMON TURKEY
Taxonomic ID9103
Entity ID2


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Ligand non-polymer