Human Factor VIII C2 Domain complexed to human monoclonal BO2C11 Fab.


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B-Cell Epitope
Epitope  
   
Complex PDB ID 1IQD
Accession Number 3DIEP0256
IEDB ID 115021
Epitope Sequence F2215; T2216; N2217; M2218; F2219; R2234; R2239; Q2241; V2242; S2269; L2270; L2271; T2272; H2334; Q2335
Starting Position2215-2219,2234,2239,2241-2242,2269-2272,2334
Ending Position2335
Epitope Type Discontinuous Epitope

Assay Information  
Assay Antigen CSMPLGMESKAISDA QITASSYFTNMFATW SPSKARLHLQGRSNA WRPQV… Coagulation factor VIII precursor (2193-2348) Homo sapiens
PDB CategoryIMMUNE SYSTEM/BLOOD CLOTTING
Keyword FACTOR VIII; C2 DOMAIN; ANTIBODY; BLOOD COAGULATION; INHIBITOR; BO2C11; IMMUNE SYSTEM/BLOOD CLOTTING COMPLEX
Antibody Residues Interacting with Antigen Open in new window      Download dimplot pdb file
Antibody Chain 1 PDB Chain B
Antibody Chain 2 PDB ChainA
Antigen PDB ChainC
Contact Area for Antigen 1074.6
Contact Area for Antibody901.3
CommentsAntigen changes conformation upon binding comparing with free antigen [PDB:1D7P]. The fold of C2 domain of factor VIII is similar in the free [PDB:1D7P] and Fab-bound (this structure) forms; however the backbone of a loop containing M2199 and F2200 of the epitope twists significantly. M2199 and F2200 of the epitope form salt bridges with D52 and D99 of the heavy chain of antibody. C2 domain of factor VIII contains binding site for von Willebrand factor (vWF) and for negatively charged phospholipids. Antibody binding to C2 domain inhibits its ability to bind negatively charged phospholipids and vWF.

Experimental Details
Method
X-RAY DIFFRACTION
Resolution
2
R-Value
Space Group
P 21 21 21
Unit Cell
Length(Å) Angle(°)
a = 64.535 α = 90
b = 73.701 β = 90
c = 112.395 γ = 90



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Source Information  
Structure Determination Method X-RAY DIFFRACTION
Source Organism Homo sapiens
Common Namehuman
Taxonomic ID9606
Source Organism Homo sapiens
Common Namehuman
Taxonomic ID9606
Entity ID2


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Ligand non-polymer