Camelid VHH Domain in Complex with Porcine Pancreatic alpha-Amylase


Tab-View Sample


B-Cell Epitope
Epitope  
   
Complex PDB ID 1KXQ
Accession Number 3DIEP0248
IEDB ID 121102
Epitope Sequence N68; P69; W74; Q78; G119; G121; A159; S160; G162; E164; Y166; V178; G179; L180; E255; H320; N362; V364; N365; E367; V369; N370 W372
Starting Position68-69,74,78,119,121,159-160,162,164,166,178-180,255,320,362,364-365,367,369-370,372
Ending Position372
Epitope Type Discontinuous Epitope

Assay Information  
Assay Antigen Pancreatic alpha-amylase precursor Sus scrofa
PDB CategoryHYDROLASE; IMMUNE SYSTEM
Keyword ALPHA 8 BETA 8; BETA BARREL; HYDROLASE; IMMUNE SYSTEM
Antibody Residues Interacting with Antigen Open in new window      Download dimplot pdb file
Antibody Chain 1 PDB Chain H
Antibody Chain 2 PDB ChainA
Antigen PDB ChainH
Contact Area for Antigen 1087.6
Contact Area for Antibody1084
CommentsThe conformational changes in the antigen upon AMB7 VHH binding with regards to the native enzyme involve the 220-225; 237-241; and 348-354 loops. For 348-354 loop; the effect was larger in the current complex with AMD9 VHH [PDB: 1KXQ] and weaker in the complexes with AMB7 [PDB: 1KXT] and AMD10 [PDB: 1KXV].

Experimental Details
Method
X-RAY DIFFRACTION
Resolution
1.6
R-Value
Space Group
P 1
Unit Cell
Length(Å) Angle(°)
a = 65.17 α = 79.31
b = 100.92 β = 72.63
c = 103.74 γ = 86.11



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Source Information  
Structure Determination Method X-RAY DIFFRACTION
Source Organism Sus scrofa
Common Namepig
Taxonomic ID9823
Entity ID1


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Ligand non-polymer