ENGINEERED HUMAN INTERLEUKIN-10 MONOMER COMPLEXED TO 9D7 FAB FRAGMENT


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B-Cell Epitope
Epitope  
   
Complex PDB ID 1LK3
Accession Number 3DIEP0242
IEDB ID 122245
Epitope Sequence F89; Y90; L91; E92; E93; V94; M95; P96; Q97; A98; E99; N100; Q101; K143; N144; A145; F146; N147; K148; L149; Q150; E151; K152; G153; I154; Y155
Starting Position89-101,143
Ending Position155
Epitope Type Discontinuous Epitope

Assay Information  
Assay Antigen Interleukin-10 precursor Homo sapiens
PDB CategoryIMMUNE SYSTEM
Keyword ANTIGEN-ANTIBODY COMPLEX; IMMUNE SYSTEM
Antibody Residues Interacting with Antigen Open in new window      Download dimplot pdb file
Antibody Chain 1 PDB Chain H
Antibody Chain 2 PDB ChainL
Antigen PDB ChainA
Contact Area for Antigen 827.3
Contact Area for Antibody782.3
CommentsThe antigen residues E75; N82; Q83; E133 form hydrogen bonds with the Fab. All the six CDR loops interact with the antigen. Fab light chain s N-terminal residue D1 forms salt bridge with the antigen K130. Comparison of this Fab/IL-10M1 complex with free [PDB: 2ILK] and sIL-10R1-bound [PDB: 1J7V] IL-10 shows that Fab binding induces conformational changes in the antigen residues 38-44; helix F residue Y137 and CD loop residues Q83; D84; P85; and D86 -- residues critical for the IL-10R1 binding.

Experimental Details
Method
X-RAY DIFFRACTION
Resolution
1.91
R-Value
0.1872
Space Group
P 1 21 1
Unit Cell
Length(Å) Angle(°)
a = 82.554 α = 90
b = 75.616 β = 96.85
c = 111.975 γ = 90



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Source Information  
Structure Determination Method X-RAY DIFFRACTION
Source Organism Rattus norvegicus
Common NameNorway rat
Taxonomic ID10116
Source Organism Rattus norvegicus
Common NameNorway rat
Taxonomic ID10116
Entity ID3


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Ligand non-polymer