Crystal Structure of the Fab fragment of a R310 antibody complexed with (R)-HNE-histidine adduct


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B-Cell Epitope
Epitope  
   
Complex PDB ID 2DDQ
Accession Number 3DIEP0172
IEDB ID 119072
Epitope Sequence (2E;4R)-4-hydroxynon-2-enal
Starting Position502
Ending Position502
Epitope Type Non peptidic

Assay Information  
Assay Antigen N-acetyl-1-[(2R)-5-hydroxy-2-pentyltetrahydrofuran-3-yl]-L-histidine
PDB CategoryIMMUNE SYSTEM
Keyword monoclonal antibody; 4-hydroxy-2-nonenal; immunoglobulin; IMMUNE SYSTEM
Antibody Residues Interacting with Antigen Open in new window      Download dimplot pdb file
Antibody Chain 1 PDB Chain H
Antibody Chain 2 PDB ChainL
Antigen PDB ChainL
Contact Area for Antigen 448.5
Contact Area for Antibody278.1
CommentsAntibody primarily binds the epitope by sandwiching between hydrophobic domains composed of several critical stacking Tyr and Phe residues. Details of epitope-antibody contacts and paratope residues are not discussed in the paper.

Experimental Details
Method
X-RAY DIFFRACTION
Resolution
2.35
R-Value
0.225
Space Group
C 1 2 1
Unit Cell
Length(Å) Angle(°)
a = 127.318 α = 90
b = 65.794 β = 118.77
c = 64.31 γ = 90


Source Information  
Structure Determination Method X-RAY DIFFRACTION


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Ligand
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