Crystal Structure of Fab CR6261 in Complex with a H5N1 influenza virus hemagglutinin.


Tab-View Sample


B-Cell Epitope
Epitope  
   
Complex PDB ID 3GBM
Accession Number 3DIEP0261
IEDB ID 97823
Epitope Sequence [GI: 224983674]: H32; Q34; D35; I36; S292; M293; P294; T319 [GI: 224983675]: D19; G20; W21; K38; T41; Q42; I45; D46; T49; V52; N53; I56
Starting Position32,34-36,292-294,319
Ending Position319
Epitope Type Discontinuous Epitope

Assay Information  
Assay Antigen Hemagglutinin Influenza A virus (A/Viet Nam/1203/2004(H5N1))
PDB Categoryviral protein / immune system
Keyword hemagglutinin; Fab; neutralizing antibodies; antibody; avian flu; Envelope protein; Fusion protein; viral protein / immune system COMPLEX
Antibody Residues Interacting with Antigen Open in new window      Download dimplot pdb file
Antibody Chain 1 PDB Chain H
Antibody Chain 2 PDB ChainL
Antigen PDB ChainA
Contact Area for Antigen 699
Contact Area for Antibody795.7
CommentsSeven hydrogen bonds are formed between the HA1 chain s residue S291 and the Fab heavy chain s residue D72; the HA1 chain s residues D19; F29; Q42; D46; and N53 and the Fab heavy chain s residues F29; S31; D72; and Y98. Five of these hydrogen bonds are similar to those in the complex CR6261 Fab with the SC1918/H1 HA [PDB: 3GBN]. The complexes also share 104 van der Waals contacts.


Experimental Details
Method
X-RAY DIFFRACTION
Resolution
2.7
R-Value
0.20586
Space Group
P 21 3
Unit Cell
Length(Å) Angle(°)
a = 202.761 α = 90
b = 202.761 β = 90
c = 202.761 γ = 90



Source Information  
Structure Determination Method X-RAY DIFFRACTION
Host OrganismTRICHOPLUSIA NI
Host Organism StrainHI5
Host Taxonomic ID 7111
PlasmidpFASTBAC-HT
  
Host OrganismTRICHOPLUSIA NI
Host Organism StrainHI5
Host Taxonomic ID 7111
PlasmidpFASTBAC-HT
  


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