CRYSTAL STRUCTURE OF THE VON WILLEBRAND FACTOR (VWF) A1 DOMAIN I546V MUTANT IN COMPLEX WITH THE FUNCTION BLOCKING FAB NMC4


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B-Cell Epitope
Epitope  
   
Complex PDB ID 1FNS
Accession Number 3DIEP0229
IEDB ID 130423
Epitope Sequence P1390; Q1391; R1392; S1394; R1395; N1396; F1397; V1398; R1399; K1493; Q1494; L1497
Starting Position1390-1399,1493-1494,1497
Ending Position1497
Epitope Type Discontinuous Epitope

Assay Information  
Assay Antigen YCSRLLDLVFLLDGS SRLSEAEFEVLKAFV VDMMERLRISQKWVR VAVVE… von Willebrand factor (1271-1467) Homo sapiens
PDB CategoryIMMUNE SYSTEM
Keyword VON WILLEBRAND FACTOR; GLYCOPROTEIN IBA (A:ALPHA) BINDING; COMPLEX (WILLEBRAND/IMMUNOGLOBULIN); BLOOD COAGULATION TYPE 2B VON WILLEBRAND DISEASE; IMMUNE SYSTEM
Antibody Residues Interacting with Antigen Open in new window      Download dimplot pdb file
Antibody Chain 1 PDB Chain H
Antibody Chain 2 PDB ChainL
Antigen PDB ChainA
Contact Area for Antigen 681.3
Contact Area for Antibody613.4
CommentsThis structure and the structure of wild-type A1 domain of vWF in complex with NCM-4 Fab [PDB: 1OAK]; as well as the structure of unliganded antigen [PDB: 1AUQ] are essentially identical; meaning that the antibody does not change the antigen conformation. While the I546V substitution that is outside the epitope makes the distant conformational effects altering the charge distribution around ß2-ß3 loo

Experimental Details
Method
X-RAY DIFFRACTION
Resolution
2
R-Value
0.172
Space Group
C 1 2 1
Unit Cell
Length(Å) Angle(°)
a = 209.396 α = 90
b = 62.673 β = 108.88
c = 73.789 γ = 90



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Source Information  
Structure Determination Method X-RAY DIFFRACTION
Source Organism Mus musculus
Common Namehouse mouse
Taxonomic ID10090
Source Organism Mus musculus
Common Namehouse mouse
Taxonomic ID10090
Entity ID2


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Ligand non-polymer