Crystal structure of 3A6 TCR bound to MBP/HLA-DR2a


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T-Cell Epitope
Epitope  
   
Complex PDB ID 1ZGL
Accession Number 3DIEP0297
IEDB ID 68846
Epitope Sequence VHFFKNIVTPRTP
Starting Position1
Ending Position13
Epitope Type Linear Epitope

Assay Information  
Assay Antigen VHFFKNIVTPRTP MBP Homo sapiens
PDB CategoryIMMUNE SYSTEM
Keyword TCR/peptide/MHC complex; IMMUNE SYSTEM
Antibody Residues Interacting with Antigen Open in new window      Download dimplot pdb file
Antibody Chain 1 PDB Chain M
Antigen PDB ChainC
CommentsThe CD4+ T cell clone was isolated from a DR2-positive MS patient with a relapsing-remitting disease course. To stabilize the TCR-MHC/Epitope complex for crystallization; the epitope was covalently attached to the N-terminus of the TCR Vß domain via an octapeptide linker. There are four complexes in the crystallographic asymmetric unit. Because of their close similarity; the analysis of the TCR-MHC interactions applies to all four complexes. Clear electron density was observed for the interface between TCR and MHC/epitope in each of the four complexes. Several loops in the MHC ß2 chain and the TCR constant domains were disordered; but these regions are distant from the TCR-MHC interface. PDB chains for each complex are: Complex I : MHC ?-chain: A; MHC ß-chain: B; epitope chain: C; TCR ?-chain: M; TCR ß-chain: P. Complex II : MHC ?-chain: D; MHC ß-chain: E; epitope chain: F; TCR ?-chain: Q; TCR ß-chain: R. Complex III : MHC ?-chain: G; MHC ß-chain: H; epitope chain: I; TCR ?-chain: S; TCR ß-chain: T. Complex IV : MHC ?-chain: J; MHC ß-chain: K; epitope chain: L; TCR ?-chain: U; TCR ß-chain: V.

Experimental Details
Method
X-RAY DIFFRACTION
Resolution
2.8
R-Value
Space Group
P 1
Unit Cell
Length(Å) Angle(°)
a = 96.48 α = 74.39
b = 97.69 β = 83.19
c = 124.02 γ = 61.55



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Source Information  
Structure Determination Method X-RAY DIFFRACTION